Synthetic modified messenger RNA for therapeutic applications

Synthetic Biology for Therapeutic Applications

Synthetic biology is a relatively new field with the key aim of designing and constructing biological systems with novel functionalities. Today, synthetic biology devices are making their first steps in contributing new solutions to a number of biomedical challenges, such as emerging bacterial antibiotic resistance and cancer therapy. This review discusses some synthetic biology approaches and ...

Exploring the Immunogenicity and Therapeutic Applications of Boranophosphate - modified RNA : siRNA and RNA Aptamers

Exploring the Immunogenicity and Therapeutic Applications of Boranophosphatemodified RNA: siRNA and RNA Aptamers by Mariam Lucila Sharaf Department of Chemistry Duke University Date:_______________________ Approved: ___________________________ Barbara Ramsay Shaw, Supervisor ___________________________ Katherine J. Franz ___________________________ Thom LaBean ___________________________ Bruce ...

A Modified Orcinol Reaction for RNA Determination

Construction of a Synthetic Messenger Encoding a Membrane Protein RNA

We have synthesized microgram quantities of a functional eucaryotic mRNA by in vitro transcription. For this purpose, we constructed a plasmid in which the Escherichia coli lactose promoter was 5' to the vesicular stomatitis virus (VSV) G protein gene (Rose, J. K., and C. J. Gallione, 1981, J. ViroL, 39:519-528). This DNA served as the template in an in vitro transcription reaction utilizing E....

Construction of a synthetic messenger RNA encoding a membrane protein

We have synthesized microgram quantities of a functional eucaryotic mRNA by in vitro transcription. For this purpose, we constructed a plasmid in which the Escherichia coli lactose promoter was 5' to the vesicular stomatitis virus (VSV) G protein gene (Rose, J. K., and C. J. Gallione, 1981, J. Virol., 39:519-528). This DNA served as the template in an in vitro transcription reaction utilizing E...